TypeError:Methode 'get' von undefined kann nicht aufgerufen werden

Ihre app.use sollte vor Ihrem app.get geschrieben werden . Die app.get wird zuerst von Express angerufen.

var mongo = require('mongodb');
var monk = require('monk');
var db = monk('localhost:27017/notepad');

var app = express();

// mongoDB
// Do all your "pre-route" use() functions first
app.use(function (req, res, next) {
   req.locals.db = db; // this is setting up db property to request
   next();
});

app.get('/users', routes.userlist);

In Ihren Routen...

var db = req.locals.db; // Instead of req.db

Oder noch besser...

var mongo = require('mongodb');
var monk = require('monk');
var db = monk('localhost:27017/notepad');

var app = express();

app.locals.db = db;

app.get('/users', routes.userlist); // Access it using req.locals.db